Systemic Inflammation Models

Preclinical CRO Services

for the Study of Systemic Inflammation

 

Systemic inflammatory diseases are triggered by dysregulated activation of immune cells. It can be frequently observed in a variety of organ transplantation cases or septic shock. Rodent model studies are helpful to decipher the disease triggering or sustaining mechanisms observed in patients.

Oncodesign Services offers access several preclinical models addressing a variety of systemic inflammation mechanisms, and provides support to the development of research models meeting your specific needs.

 

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Typical readouts for systemic inflammation

  • Clinical scoring
  • Body weight
  • Body temperature (for shock models)
  • Survival
  • Histopathology
  • Biomarker/drug monitoring
  • Gene expression in relevant tissues, by qPCR/dPCR
  • Immune phenotyping

Discover Oncodesign Services offers for systemic inflammation diseases

  • In vitro assays
    • LPS or TNFa stimulation of human primary monocytes
    • TLR ligand stimulation on human PBMC and human whole blood
    • TLR ligand stimulation on mouse RAW264.7 macrophage cell line
  • Systemic Inflammation models List
    • Graft-versus-host disease, allogeneic, with Balb/c (H2d) irradiated mice or with C57b6D2F1 (H2d) mice.
    • Graft-versus-host disease, xenogeneic, in NOG mice.
    • Systemic inflammatory response syndrome, induced by TNFa, causing hypothermia.
    • Systemic IFNg response, IL-12/IL-18-induced.
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Case studies

  • #1 Xenogeneic GvHD

    Graft-versus host disease (GvHD) is a major inflammatory complication after hematopoietic cell transplantation, with around 50% of patients undergoing GvHD.  Xenogeneic GvHD models use human donor cells transplanted into NOG immuno-deficient mice, with monitoring over 30+ days. Cyclosporine is typically used as a reference compound.

  • #2 GVHD xenogeneic mouse model

    NOG mice are transplanted IP with human PBMCs. The analysis of human T cell population performed by flow cytometry is used for longitudinal monitoring of human (hCD45+) leucocytes frequency in blood. Human immune infiltrates are also observed in tissues such as gastro-intestinal tract.