Preclinical CRO Services
for Inflammatory Diseases of the Liver
Acute and chronic inflammatory diseases of the liver tract can be triggered by several causes such as exposure to toxic chemicals, diet or immune dysregulation. Rodent models of liver inflammation are helpful to decipher the disease triggering or sustaining mechanisms observed in patients.
Oncodesign Services offers CRO services for several preclinical models addressing a variety of liver diseases, and provides CRO support for de novo development of new research models recently described in literature.
Typical readouts in liver inflammation
- NASH score
- Neutrophil infiltration
- Serum markers of liver injury: LDH, ALT, AST
- Inflammation markers
- Immune cell infiltrates
- Ballooning degeneration of hepatocytes
- Apoptosis markers or necrotic histology
- Gene expression in liver, by qPCR/dPCR
- Biomarker / drug monitoring
Case studies in liver inflammation
The CCl4 model of liver fibrosis is a classic model widely used in industry. Juvenile mice are given repeated doses of CCl4.Liver fibrosis then typically appears at 6 weeks old.
This model is useful for therapies aiming to modulate collagen depositions and expression of metalloproteases. ALK5 (TGFb receptor I) inhibition, a well-known anti-fibrotic mechanism, decreases collagen induction and is used as a reference compound.