Proof of concept in preclinical pharmacology with inflammation models
Oncodesign Services has a big collection of preclinical inflammatory and autoimmune models, covering diseases of the skin, gut, kidneys, liver, uterus and more. We offer studies as individual or as part of integrated packages covering all aspects of the preclinical development. Because of the shared mechanism of action of many inflammatory indications, bundling lead optimization with cell-based screening and in vivo pharmacology delivers fast cycle times and enable quick switching between indications of relevance.
Identification and setup of new experimental models of interest meeting specific requirements is highly needed for the development of drugs in inflammatory disease areas . It is critical to customize new inflammation models according to specific targets and drug of interest. Oncodesign Services is happy to provide custom-made model development support for these kinds of specific requests.
Typical readouts for inflammatory and autoimmune mouse models include:
- Clinical monitoring and scoring
- Lung mechanical assessments (FlexiVent system – SCIREQ)
- Clinical biochemistry
- Organ histology
- Multiplex cytokine profiling
- Immune cell phenotyping (FACS)
- T cell responses (ELISPOT, proliferation, FACS)
- DMPK/PD
- In vivo pharmaco-imaging
- Gene biomarker expression monitoring
A typical study design with inflammation models
In Vitro Assays & biomarker analytics for inflammation studies
Immortalized human cell lines and fresh human samples are available for in vitro study of an inflammatory Mode of Action (MOA). Examples include fresh human skin, PBMCs, hepatocytes, immortalized HPASMC (Human Pulmonary Arterial Smooth Muscle Cells).
Read more about our in vitro platform.
Inflammation models typical require cytokine response & immune cell analysis (systemic and in situ). We use all commercially available flow cytometry reagents as well as cytokine kits and panels, and we can customize the panels depending on your specific requirements.
A deep expertise with humanized inflammation models
Oncodesign Services offers human xenografts on mice for humanized inflammatory models, including colitis models and GvHD models. Inflammatory mouse models with human immune systems have been established to study development, maintenance and function of human hematopoietic cells. Please ask us whether a humanized version of your favorite inflammatory model is right for your preclinical program:
- GVHD xenogeneic mouse model
- NOG mice are transplanted IP with human PBMCs
In these models, monitoring the populations of immune cells is a crucial readout to monitor the progression of the disease. The analysis of human T cell population performed by flow cytometry is used for longitudinal monitoring of human (hCD45+) leucocyte frequency in blood. Human immune infiltrates are also observed in tissues such as gastro-intestinal tract.
A validated Endometriosis mouse model
Recently, Oncodesign Services has built a mouse model to investigate endometriosis, a chronic inflammatory disease affecting millions of women worldwide.
Fibrosis
Fibrosis is a common sequalae of many repair mechanisms resulting from inflammatory cascades. Fibrosis is a serious concern as it leads to organ function defects. Oncodesign Services offers several fibrosis models in the lung (bleomycin), skin (bleomycin, TopoI) and liver (CCl4, NASH) therapeutic areas.
Do you have a request for an inflammation project ? Contact-us!
